DSIP 10mg

What is DSIP ?

Delta sleep-inducing peptide (DSIP) is a nonapeptide (Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) first isolated from rabbit cerebral venous blood. Research has examined its reported effects on sleep architecture, stress hormone regulation and circadian rhythms, with human evidence remaining limited. This 10mg presentation is the higher-mass research format of our 5mg vial.

DSIP 10mg holds the same delta sleep-inducing peptide as our 5mg vial, at twice the mass. It is a nonapeptide, Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, with a free acid C-terminus, a molecular weight of 848.8 g/mol and CAS number 62568-57-4. Its international nonproprietary name is emideltide. Schoenenberger and Monnier isolated it from rabbit cerebral venous blood (Proc Natl Acad Sci U S A, 1977). They named it for the delta-wave EEG activity its intraventricular infusion appeared to produce in recipient animals.

Much of its biology is still open. No receptor has been identified, no gene encoding it has been isolated, and whether it occurs naturally is disputed. Kovalzon and Strekalova (J Neurochem, 2006) called it a still unresolved riddle.

DSIP Research Applications

• Sleep : in a 1977 double-blind test in 58 rabbits, only synthetic DSIP of nine related peptides gave specific EEG enhancement.
• Replication : Kovalzon and Strekalova (J Neurochem, 2006) call the sleep-factor case poorly documented, noting that some DSIP analogues, not DSIP itself, promoted slow-wave sleep.
• Stress axis : in vitro, DSIP inhibited corticotropin-releasing factor-induced ACTH release from rat anterior pituitary tissue (Okajima and Hertting, Horm Metab Res, 1986). The effect peaked at 10 nM, and basal ACTH did not change.
• Circadian rhythm : Graf and Kastin (Neurosci Biobehav Rev, 1984) catalogued effects on circadian and locomotor patterns and on circulating hormone levels.

The primary literature is old, uneven and in places contradictory. DSIP is best treated as a compound for hypothesis testing and replication, not a tool with a known mechanism.

DSIP 10mg vs DSIP 5mg: Choosing the Research Format

Both vials hold the identical compound, with the same sequence, CAS number and lyophilised form. The difference is logistics. The 5mg vial suits pilot work and single assays. The 10mg vial puts twice the mass in one container, so a long series needs fewer vials and fewer reconstitutions, and vial-to-vial variation matters less. That headroom suits replication work, which the uneven DSIP literature needs.

Research Overview

Delta sleep-inducing peptide (DSIP) is a nonapeptide first isolated from rabbit cerebral venous blood, and it is the compound behind Peptaro's DSIP 10mg vial. The sequence is Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, an unusually acidic nine-residue peptide with a molecular weight of 848.8 g/mol, molecular formula C₃₅H₄₈N₁₀O₁₅ and CAS number 62568-57-4. It was isolated by the Schoenenberger and Monnier group in Basel and reported in 1977, when the team showed that material recovered from rabbits during electrically induced slow-wave sleep enhanced delta and spindle electroencephalogram activity after intraventricular infusion (Schoenenberger and Monnier, Proc Natl Acad Sci U S A, 1977). This 10mg presentation is the higher-mass research format of our 5mg vial: the same compound, supplied at twice the peptide mass per vial, for laboratories running extended assay series.

Research has examined DSIP across three main strands: sleep architecture, stress hormone regulation and circadian rhythms. The earliest sleep work reported enhanced delta-wave sleep in several species, and Graf and Kastin's review of the field (Neurosci Biobehav Rev, 1984) catalogued those early reports alongside effects on circadian and locomotor patterns and on circulating hormone levels. On the neuroendocrine side, in-vitro work reported that DSIP inhibited corticotropin-releasing factor-induced ACTH secretion from rat anterior pituitary tissue, placing it in the hypothalamic-pituitary-adrenal literature (Okajima and Hertting, Horm Metab Res, 1986).

The honest caveat belongs up front, because DSIP is a compound whose name promises more than the literature delivers. No specific DSIP receptor has ever been identified, no gene encoding the peptide has been found in any sequenced genome, and the original sleep-induction findings have proved difficult to replicate consistently; Kovalzon and Strekalova summarised the field in the Journal of Neurochemistry in 2006 under the title 'a still unresolved riddle'. Human evidence remains limited. Early human studies reported effects on disturbed sleep (Schneider-Helmert and Schoenenberger, Neuropsychobiology, 1983), but a double-blind matched-pairs study in 16 chronic insomniac patients found weak, partly confounded effects and concluded that short-term treatment was unlikely to offer major therapeutic benefit (Bes et al., Neuropsychobiology, 1992).

DSIP is not an approved medicine in any jurisdiction and is supplied as lyophilised powder for in-vitro laboratory research use only, with no dosing information and no human-use claims. The 10mg vial is supplier-tested, with the certificate published on this page, and independent third-party verification is pending.

Storage and Reconstitution

Move vials to the freezer on arrival and keep them dark and dry. Keep the solution in a light-protected container. Aliquot at reconstitution: thawing a whole 10mg stock again and again degrades the tryptophan in everything left. Tryptophan oxidation shows up as a new RP-HPLC peak after long or light-exposed storage.

Vials are sold on their own, without bacteriostatic water for mixing or other supplies.

How to reconstitute and handle

• The N-terminal tryptophan is the oxidation- and light-sensitive residue: work away from direct sunlight, keep solutions cold, and minimise headspace and stopper-open time.
• Handle under aseptic laboratory conditions. With twice the peptide mass per vial, prepare single-use aliquots at reconstitution so repeated freeze-thaw cycles do not degrade the remaining stock.

For the full method, see the peptide reconstitution guide and the reconstitution calculator.

Research References

• Delta sleep-inducing peptide (DSIP): a still unresolved riddle (J Neurochem, 2006)
• Delta-sleep-inducing peptide (DSIP): a review (Neurosci Biobehav Rev, 1984)

Published research on DSIP is indexed on PubMed. See our full UK buyer's guide for DSIP 10mg.